Method for producing monoclonal antibodies specific to hair foll

Details Method for producing monoclonal antibodies specific to hair foll Method for producing monoclonal antibodies specific to hair foll

1994-11-10

1997-10-07

Loring, Susan A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 7021, 43524027, 5303882, C12N 1502, C12N 512, C12P 2108, C07K 1628

Patent

active

056747195

ABSTRACT:
This invention provides a method for preparing hybridomas, which include the steps of: quickly and deeply freezing hair-bulbs of follicle isolated from a human or an animal of species A; immunizing an animal of species B by subcutaneously administering the hair-bulbs after being thawed out to the animal; re-immunizing the animal by intravenously and intraperitoneally administering liquid and solid components of hair-bulbs obtained by centrifugation of minced thawed hair-bulbs to the animal; fusing spleen cells or lymphoid cells isolated from the immunized animal with myeloma cells derived from an animal of species B or C, and cloning fusion cells which produce antibodies capable of specifically binding to the hair follicle of human or animal of species A. The hybridomas prepared by the above methods can produce monoclonal antibodies specifically recognizing hair follicle tissues, and these antibodies enable functional analysis of hair follicle in addition to development of new diagnostic and therapeutic means for abnormal hair development, alopecia, etc.

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