Method for producing microbial cells and use thereof to produce

Details Method for producing microbial cells and use thereof to produce Method for producing microbial cells and use thereof to produce

1979-03-27

1981-05-05

Penland, R. B.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435190, 435247, 435822, 435858, C12N 1500

Patent

active

042660343

ABSTRACT:
Disclosed are newly discovered and isolated methylotrophic microorganism strains and their natural and/or artificial mutants which grow well under aerobic conditions in a culture medium in the presence of methane as the major carbon and energy source. The methane-grown microbial cells possess a high content of protein and can be utilized as such as feedstuffs. The methane-grown microbial cells or enzyme preparations thereof are also useful in converting C.sub.1 -C.sub.6 alkanes to alcohols, particularly methane to methanol, C.sub.3 -C.sub.6 alkanes to the corresponding C.sub.3 -C.sub.6 sec. alcohols and methyl ketones, C.sub.3 -C.sub.6 sec. alcohols to the corresponding methyl ketones, cyclic hydrocarbons to cyclic hydrocarbyl alcohols (e.g., cyclohexane to cyclohexanol) C.sub.2 -C.sub.4 alkenes selected from the group consisting of ethylene, propylene, butene-1 and butadiene to 1,2-epoxides, styrene to styrene oxide, and converting other oxidizable substrates to oxidized products. The newly discovered and isolated methylotrophic microorganism strains may be aerobically grown on a plurality of methyl-radical donating carbon-containing compounds, such as methanol, methylamine, methyl formate, methyl carbonate, dimethyl ether, etc., to produce microbial cells or enzyme preparations capable of aerobically converting C.sub.3 -C.sub.6 linear secondary alcohols to the corresponding methyl ketones.

REFERENCES:
patent: 3326771 (1967-06-01), Leavitt
patent: 3344037 (1967-09-01), Leavitt
patent: 3930947 (1976-01-01), Morinaga
Colby et al., "The Soluble Methane Monooxygenase of Methylococcus Capsulatus", Biochem. J., vol. 165, (1977), pp. 395-402.
Nesterov et al., "Activity of Methane Oxidizing Bacterium in the absorbed state", Chemical Abstracts, vol. 84 (1976), Abstract No. 27940c.
Thomson et al., "Acetone Production by Methylobacterium", Chemical Abstracts, vol. 85 (1976), Abstract No. 156225t.

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