Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2008-04-29
2008-04-29
Lilling, Herbert J. (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S069100, C435S106000, C435S110000, C435S252310, C435S252500
Reexamination Certificate
active
10974442
ABSTRACT:
Methods for producing high molecular weight poly-gamma-glutamic acid (PGA). The PGA is produced by fermentation, and purified by use of tangential flow filtration, followed by diafiltration, as necessary, to yield a product of the desired purity. Product obtained may be of very high purity using all the prescribed purification steps. Product of this purity is suitable for in vivo medical applications. Other applications, such as food or agricultural, may utilize lower purity levels, and hence do not require all the purification steps specified.
REFERENCES:
patent: 6533938 (2003-03-01), Dilorio et al.
Louis Richard Stock II, Theological Characterization Of (Poly-y) Glutamic Acid Fermentations, Aug. 1996, 92 pages.
K. Kimura and Y. Itoh,Appl. Environ. Microbiol.,69:2491-2497. 2003.
F.A. Troy.J. Biol. Chem.248: 316-324. 1973.
F.A. Troy.J. Biol. Chem.248: 305-315. 1973.
L.R. Stock. “Mixing and mass transfer studies of the fermentation product (poly)-y-glutamic acid.” Ph.D. Dissertation, Worcester Polytechnic Institute. pp. 1-115. 2000.
Prescott Albert G.
Stock, II Louis R.
Cresent Innovations, Inc.
Dingman Brian M.
Lilling Herbert J.
Mirick, O'Connell, DeMollie & Lougene, LLP
Schray Kerri P.
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