Method for producing L-tryptophan by fermentation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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4351723, 435253, 435317, C12P 1322, C12N 1500, C12N 120, C12N 100

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045886873

ABSTRACT:
A method for producing L-tryptophan by fermentation in high yields employing a microorganism of the genus Bacillus, which has been constructed by a gene splicing technique by incorporating a recombinant plasmid DNA containing an inserted DNA containing fragment controlling resistance to tryptophan-antagonists into a recipient strain of the genus Bacillus. The incorporated DNA fragment is a mutant of the genus Bacillus which was obtained from resistant to tryptophan-antagonists.

REFERENCES:
patent: 4302544 (1981-11-01), Young et al.
patent: 4363875 (1982-12-01), Akashiba et al.
patent: 4371614 (1983-02-01), Anderson et al.
patent: 4430434 (1984-02-01), Sanders et al.
Chemical Abstracts, vol. 95, p. 339, (1981), Abstract No. 57898q: Okunev, O. V. et al, "Obtaining Hybrid Plasmids Containing Bacillus subtilis Genes".
Keggins et al, Proc. Natl. Acad. Sci., vol. 75, No. 3, pp. 1423-1427 (1978), "Molecular Cloning of Genetically Active Fragments of Bacillus DNA in Bacillus subtilis and Properties of the Vector Plasmid pUB110".
Methods in Enzymology, vol. 68, Recombinant DNA; Wu, R. ed., Academic Press, New York, pp. 342-357 (1979).

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