Method for producing L-threonine, and plasmid and microorganism

Details Method for producing L-threonine, and plasmid and microorganism Method for producing L-threonine, and plasmid and microorganism

1988-10-14

1992-10-06

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435840, C12P 1308

Patent

active

051531232

ABSTRACT:
A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.
According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.

REFERENCES:
patent: 3494830 (1970-02-01), Nakayama et al.
patent: 3582471 (1971-06-01), Shiio et al.
patent: 5019503 (1991-05-01), Terasawa et al.
Gottschalk, Bacterial Metabolism, p. 41, 1979.

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