Method for producing L-lysine or L-arginine by using...

Details Method for producing L-lysine or L-arginine by using... Method for producing L-lysine or L-arginine by using...

2011-08-09

2011-08-09

Fronda, Christian (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S252300, C435S252330, C435S320100, C530S350000, C536S023100

Reexamination Certificate

active

07993885

ABSTRACT:
A DNA encoding a variant of a protein having a loop region and six hydrophobic helixes which is involved in excretion of L-lysine to outside of a cell is described, wherein the DNA encodes a mutant protein which does not contain the loop region that is present in the wild-type protein. The mutant protein facilitates excretion of L-lysine, L-arginine, or both to the outside of the cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium. Specifically, lysE24 is introduced into a methanol assimilating bacterium such asMethylophilusbacteria which results in improved L-amino acid productivity, especially production of L-lysine and L-arginine.

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