Method for producing L-glutamic acid by fermentation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S041000, C435S106000, C435S252300, C435S252320, C435S320100, C435S471000, C530S350000, C536S023200

Reexamination Certificate

active

06852516

ABSTRACT:
A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

REFERENCES:
patent: 9918228 (1999-04-01), None
Seffernick et al. , J. Bacteriol. 183(8):2405-2410, 2001.*
Witkowski et al. , Biochemistry 38:11643-11650, 1999.*
Broun et al., Science 282:1315-1317, 1998.
Van de Loo et al., Proc. Natl. Acad. Sci. 92:6743-6747, 1995.
Petra G. Peters-Wendisch et al., Microbiology, vol. 144, pp. 915-927, “Pyruvate Carboxylase from Corynebacterium Glutamicum: Characterization, Expression and Inactivation of the PYC Gene” 1998.
Petra G. Peters-Wendisch, et al., Microbiology, vol. 143, pp. 1095-1103, “Pyruvate Carboxylase as an Anaplerotic Enzyme in Corynebacterium Glutamicum” 1997.
M. A. G. Koffas, et al., Appl. Microbiol Biotechnol, vol. 50, pp. 346-352. “Sequence of the Corynebacterium Glutamicum Pyruvate Carboxylase Gene”, 1998.
Bork, Genome Research, 10:348-400, 2000.

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