Method for producing L-amino acids

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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435 691, 435 711, 435170, 4351721, 4351723, 435115, 4352523, 43525232, 435320, 435840, 536 27, 935 6, 935 9, 935 22, 935 29, 935 59, 935 60, 935 61, 935 66, 935 72, C12N 1552, C12N 121, C12N 1574, C12P 1308, C12P 1322

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049802852

ABSTRACT:
A method for producing an L-amino acid, which comprises inserting a gene which codes for an enzyme which is utilized on the route of biosynthesis of an L-amino acid product into one of at least two plasmid vectors which have compatible replicating origins different from each other, inserting a second gene which codes for an enzyme different from the first enzyme on the route of biosynthesis of the L-amino acid into a second of the plasmid vectors; introducing the thus obtained recombinant plasmids into a strain of Coryneform bacteria; and culturing the thus transformed strain which is capable of producing the L-amino acid, said two enzymes being highly rate determining enzymes for the biosynthesis of L-amino acid.

REFERENCES:
patent: 4371614 (1983-03-01), Anderson et al.
patent: 4500640 (1985-03-01), Katsumata et al.
patent: 4757009 (1988-07-01), Sano et al.

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