Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1984-07-25
1987-04-14
Wiseman, Thomas G.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
4351723, 435317, 47 58, 935 30, 935 56, 935 64, 935 67, C12N 1500, C12N 100
Patent
active
046580824
ABSTRACT:
The invention discloses an in vivo method for transforming and regenerating intact plants. According to the method, a plant (P) is infected with an infectious microbial agency comprised of: (1) virulence functions, (2) oncogenic factors capable of inducing a shoot-bearing shooty tumor on plant (P), and (3) a carrier vector containing engineered heterologous transfer DNA capable of being integrated into the nuclear DNA of plant (P) cells. Infected plant (P) is maintained until a shoot-bearing shooty tumor develops at or near the infection site. Those shoots, or progeny thereof, that contain transformed cells having heterologous transfer DNA integrated into their genomes are the selected and utilized to produce whole plants that contain cells having heterologous transfer DNA integrated into their genomes.
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Margossian Linda J.
Simpson Robert B.
Atlantic Richfield Company
Maurey Karen
Wiseman Thomas G.
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