Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof
Patent
1993-09-13
1998-06-09
Caputa, Anthony C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Virus or component thereof
4242091, 4242781, 4242801, 424816, 530396, A61K 39145, C07K 1411
Patent
active
057629390
ABSTRACT:
A method of preparing a recombinant influenza vaccine using DNA technology is provided. The resulting vaccine is a trivalent influenza vaccine based on a mixture of recombinant hemagglutinin antigens cloned from influenza viruses having epidemic potential. The recombinant hemagglutinin antigens are full length, uncleaved (HA0), glycoproteins produced from baculovirus expression vectors in cultured insect cells and purified under non-denaturing conditions. The process for cloning influenza hemagglutinin genes from influenza A and B viruses uses specially designed oligonucleotide probes and PCR. The cloned HA genes are then modified by deletion of the natural hydrophobic signal peptide sequences and replacing them with a new baculovirus signal peptide. A general approach for the efficient extraction and purification of recombinant HA protein produced in insect cells is also disclosed which can be adapted for the purification of rHA proteins from A sub-types and B type influenza viruses. The procedure produces substantially pure rHA which is a biologically active hemagglutinin, non-denatured, and suitable as a component in human or other animal influenza vaccines.
REFERENCES:
patent: 4289690 (1981-09-01), Pestka et al.
patent: 4659669 (1987-04-01), Kleid et al.
patent: 4752473 (1988-06-01), Nayak et al.
patent: 4920213 (1990-04-01), Dole et al.
Clements, "Influenza Vaccines", in Vaccines New Approaches to Immunological Problems, Ed. R.W. Ellis pp. 129-150.
Pennock et al. Mol. Cell Biology 4(3):399-406 1984.
Carr, C.M., et al., "A Spring-loaded Mechanism for the Conformational Change of Influenza Hemagglutinin", Cell 73:823-832 (May 1993).
Davis, A.R., et al, "Construction and characterization of a bacterial clone containing the hemagglutinin gene of the WSN strain (HON1) of influenza virus", Gene, 10:205-218 (1980).
Eldridge, J.H., et al., "Biodegradable Microspheres: Vaccine Delivery System for Oral Immunization", Current Topics in Microbiology and Immunology, 146:59-66 (1989).
Goodman-Snitkoff, G., et al., "Role of Intrastructural/Intermolecular Help in Immunization With Peptide-Phospholipid Complexes", J. Immunol., 147:410-415 (1991).
Harding, C.V. and H.J. Geuze, "Antigen processing and intracellular traffic of antigens and MHC molecules", Current Opinion in Cell Biology, 5:596-605 (Aug. 1993).
Johansson, B.E., et al, "Purified Influenza Virus Hemagglutinin and Neuraminidase Are Equivalent in Stimulation of Antibody Response but Induce Contrasting Types of Immunity to Infection", J. Virology, 63:1239-1246 (1989).
Kreuter, J., "Nanoparticles--Preparation and Applications" in Microcapsules and Nanoparticles in Medicine and Pharmacology, M. Donbrow, ed., CRC Press, Boca Raton, FL, pp. 125-148 (1992).
Maniatis, T., et al., "Large-Scale Isolation of Plasmid DNA", Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Lab., Cold Spring, N.Y., pp. 86-96 and 366-367 (1982).
Miller, M.D., et al., "Vaccination of Rhesus Monkeys with Synthetic Peptide in a Fusogenic Proteoliposome Elicits Simian Immunodeficiency Virus-specific CD8.sup.+ Cytotoxic T Lymphocytes", J. Exp. Med., 176:1739-1744 (1992).
Murphy, B.R. and R.G. Webster, "Influenza Viruses", in Fields Virology, Second Edition, Fields,B.N., et al., eds., Raven Press, New York, pp. 1179-1239 (1990).
Murphy, B.R. and R.G. Webster, "Orthomuxoviruses", in Fields Virology, Second Edition, Fields, B.N., et al., eds., Raven Press, New York, pp. 1091-1152 (1990).
Ogra, P.L., et al., "Clinical and Immunologic Evaluation of Neuraminidase-Specific Influenza A Virus Vaccine in Humans", J. Infect. Dis., 135:499-506 (1977).
Powers, D.C., et al., "Effect of Age on Cytotoxic T Lymphocyte Memory as well as Serum and Local Antibody Responses Elicited by Inactivated Influenza Virus Vaccine", J. Inf. Dis., 167:584-592 (Mar. 1993).
Rajakumar, A., et al, "Sequence of an influenza virus hemagglutinin determined directly from a clinical sample", Proc. Natl. Acad. Sci. USA, 87:4154-4158 (1990).
Rosen, L., "Hemagglutination with Animal Viruses", in Fundamental Techniques in Virology, K. Habel and N.P. Salzman, eds., Academic Press, New York, pp. 276-287 (1969).
Smith, G.E., et al., "Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector", Mol. and Cell. Biol., 3:2156-2165 (1983).
Update: Influenza Activity--United States and Worldwide, and Composition of the 1992-1993 Influenza Vaccine, Morbidity and Mortality Weekly Report., U.S. Department of Health and Human Services, Public Health Service, 41:315-323 (1992).
Update: Influenza Activity--United States, 1992-93 Season, Morbidity and Mortality Weekly Report, U.S. Department of Health and Human Services, Public Health Service, 42:51-53 (Jan. 1993).
Update: Influenza Activity--United States, 1992-93 Season, Morbidity and Mortality Weekly Report, U.S. Department of Health and Human Services, Public Health Service, 42:131, 137-138 (Feb. 1993).
Wang, M., et al, "Extensive Heterogeneity in the Hemagglutinin of Egg-Grown Influenza Viruses from Different Patients", Virol; ., 171:275-279 (1989).
Weiss, W., et al, "Structure of the influenza virus hemagglutinin complexed with its receptor, sialic acid" Nature, 333:426-431 (1988).
Wilson, I.A., "Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolutoin", Nature, 289:366-378 (1981).
Stanley et al. Virology 56:640-45 1973.
Possee, R.D. Virus Research 5C17:43-59 1986.
Kuroda et al. The EMBO Journal 5(6):1359-1365 1986.
Matsuura et al. J. Gen. Virol. 68:1233-1250 1987.
Luckow et al. Bio/Technology 6:47-55 1988.
Hackett Craig Stanway
Smith Gale Eugene
Volvovitz Franklin
Wilkinson Bethanie Eident
Caputa Anthony C.
Kowalski Thomas J.
MG-PMC, LLC
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