Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-03-08
2005-03-08
Brusca, John S. (Department: 1631)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024300
Reexamination Certificate
active
06864049
ABSTRACT:
The invention discloses a method for producing a hybridization complex whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules, consisting in contacting a first nucleic acid molecule with a second nucleic acid molecule under conditions enabling the formation of a specific hybridization complex. The invention is characterized in that at least one of the four types of bases contained in the sequence of the first and/or of the second nucleic acid molecule is a modified base having pairing properties close to those of the natural base it replaces, such that at least one of the two types of the base pair contained in the composition of the complex comprises a modified base and in that the totality of the base pairs contained in the composition of the said complex has a like stability.
REFERENCES:
patent: 5844110 (1998-12-01), Gold
patent: WO 93 05175 (1993-03-01), None
Melchior W.B. et al., “Alteration of the Relative Stability of DA.DT and DG.DC Base Pairs in DNA,”Proceedings of the National Academy of Sciences of the USA, vol. 70, No. 2, pp. 298-302, Feb. 1973.
Wood W. I. et al., “Base Composition-Independent Hybridization in Tetramethylammonium Chloride: A Method for Oligonucleotide Screening of Highly Complex Gene Libraries,”Proceedings of the National Academy of Sciences of USA, vol. 82, No. 3, pp. 1585-1588, Mar. 3, 1985.
Nguyen H-K, et al., “Studies Towards the Design of a Modified GC Base Pair with Stability Similar to that of the AT Base Pair,”Tetrahedron Letters, vol. 38, No. 23, pp. 4083-4086, Jun. 9, 1997.
Nguyen H., et al., “Modification of DNA Duplexes to Smooth Their thermal Stability Independently of Their Base Content for DNA Sequencing by Hybridization,”Nucleic Acids Research, vol. 25, No. 15, pp. 3059-3065, Aug. 1, 1997.
Asseline Ulysse
Bonfils Edwige
Dupret Daniel
Durand Maurice
Maurizot Jean-Claude
Appligene-Oncor S.A.
Brusca John S.
Feit Irving
Hoffmann & Baron , LLP
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