Method for producing human leukocyte interferon alpha-2

Details Method for producing human leukocyte interferon alpha-2 Method for producing human leukocyte interferon alpha-2

1985-07-03

1987-07-14

Hazel, Blondel

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435811, 424 85, C12P 2100, A61K 4502

Patent

active

046802606

ABSTRACT:
A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudomonas species VG-84 carrying a plasmid pVG3 with an inserted gene of human leukocyte interferon alpha-2, said strain being deposited in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1742; said strain being cultivated in a nutrient medium, containing the sources of carbon and nitrogen, mineral salts and growth stimulants, under aeration in the presence of antibiotics, i.e., streptomycin or tetracycline, or a mixture of both, followed by isolation and purification of the end product.

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