Method for producing human lactoferrin in plant cell culture

Details Method for producing human lactoferrin in plant cell culture Method for producing human lactoferrin in plant cell culture

2006-10-10

2006-10-10

Collins, Cynthia (Department: 1638)

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

Plant cell or cell line, per se, contains exogenous or...

C435S414000, C435S252200, C435S252300

Reexamination Certificate

active

07118914

ABSTRACT:
The present invention relates to a method for producing target protein in plant cell culture, more particularly to the method for producing target protein comprising the steps of i) constructing vector containing SWPA2 promoter and gene for the target protein, ii) introducing said vector intoAgrobacterium, iii) transforming saidAgrobacteriuminto plant cell, and iv) mass-producing target protein using said transformed plant cells. The method of the present invention can mass-produce target proteins such as human lactoferrin. Moreover, the transformed plant can also be used to make health foods when medicinal plant is used as a host cell.

REFERENCES:
patent: 6096546 (2000-08-01), Raskin
patent: WO 01/31018 (2001-05-01), None
Salmon V. et al. Production of human lactoferrin in transgenic tobacco plants. Protein Expr Purif. Jun. 1998; 13(1):127-35.
Gallie D. et al. The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation. Gene. Nov. 20, 1995; 165(2):233-8.
Choi Y. et al. Rapid and efficient Agrobacterium-mediated transformation of Panax ginseng by plasmolyzing pre-treatment of cotyledons. Plant Cell Rep. (2001) 20:616-621.
Mitra, et al., Expression of a Human Lactoferrin cDNA in Tobacco Cells Produces Antibacterial Proteins(s), Plant Physiol. 106:977-981, 1994.
Chong, et al., Expression of full-length bioactive antimicrobial human lactoferrin in potato plants, Transgenic Research 9: 71-78, 2000.
Salmon, et al., Production of Human Lactoferrin in Transgenic Tobacco Plants, Protein Expression and Purification 13, 127-135, 1998.
Arakawa, et al., Improvements in Human Health Through Production of Human Milk Proteins in Transgenic Food Plants, edited by Shahidi, et al., Chemicals via Higher Plant Bioengineering, Kluwer Academic/Plenum Publishers,NY, 149—149, 1999.

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