Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2005-02-01
2005-02-01
Mehta, Ashwin (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C435S468000, C800S287000
Reexamination Certificate
active
06849779
ABSTRACT:
The present invention provides novel DNA constructs encoding high methionine zein proteins, the expression of which is not negatively regulated by the dzr1 regulatory protein. The constructs of the invention comprise a δ-zein coding region operably linked to a promoter and a 3′ UTR which has been modified so as to be devoid of any binding sites for the dzr1 regulatory protein. Preferably, the entire 3′ UTR is replaced by a heterologous sequence that does not contain any dzr1 binding sites. Transgenic corn plants comprising the DNA constructs of the invention are also provided. These plants consistently produce high methionine corn seeds.
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Lai Jinsheng
Messing Joachim
Mehta Ashwin
Rutgers The State University of New Jersey
Woodcock & Washburn LLP
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