Method for producing heterologous bi-specific antibodies

Details Method for producing heterologous bi-specific antibodies Method for producing heterologous bi-specific antibodies

1996-11-29

1999-08-31

Chan, Christina Y.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 702, 435328, 5303873, 5303881, C12P 2104

Patent

active

059453117

ABSTRACT:
In a method for producing heterologous bi-specific antibodies, a quadroma is provided which is fused from hybridromas one of which generates antibodies that have an affinity to the binding site of protein A and another of which generates antibodies that have a weaker or no affinity to the binding domain of protein A, by multiplying and cultivating the quadromas and by eluting the bi-specific antibodies in a pH range at least 0.5 units above the pH value at which the antibodies with greater affinity to the binding domain of protein A are still bonded.

REFERENCES:
Bjorck et al., J. of Immunology, vol. 133: 969-974, 1984, Aug., 1984.
Lindhofer et al. J. Immunology vol. 155: 219-225, 1995.
S. Ferrini et al, "Targeting of T-Lymphocytes Against EGF-Receptor.sup.+ Tumor Cells by Bispecific Monoclonal Antibodies: Requirement of CE3 Molecule Cross-Linking For T-Cell Activation.", International Journal of Cancer, vol. 55, No. 6, Dec. 2 1993, pp. 931-937.
V. Lebegue et al., "Producti Onet Caracterisation D,Anticorps Monoclonaux Hybrides Presentant Une Double Isotyple IgG1/IgG3.", Comptes Rendus de l'Academie des Sciences, vol. 310, No. 9, Apr. 26, 1990, Paris, pp. 377-382.
M.Clark et al., "T-Cell Killing of Target Cells Induced by Hybrid Antibodies: Comparison of Two Bispecific Monoclonal Antibodies.", Journal of the National Cancer Institute, vol. 79, No.6, 12/87, MD, pp. 1393-1401.

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