Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1998-06-15
2000-11-14
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
424 69, 435171, 4352551, 4352552, 43525521, 4352554, 4352557, 435911, 435921, 435940, A61K 7035, C12N 100, C12N 114, C12P 102, C12P 1904
Patent
active
061468576
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to the field of cosmetology, particularly the production of primary materials for cosmetics, and has for its object a process for the production of glycogens or extracts rich in glycogens from yeast cells, as well as the use of this glycogen or these extracts in cosmetic compositions and the resulting compositions.
BACKGROUND OF THE INVENTION
It is known that glycogen, a polysaccharide comprised of glucose and energetic reserve of living cells, is particularly abundant in yeast cells.
There are already known different processes for disintegration and treatment of yeast cells for the production of proteins, particularly recombinant ones, comprising the disintegration of said cells, the separation of the cellular debris, the recovery of the proteins and the purification of these latter.
Nevertheless, these known processes do not permit a preferential extraction of glycogens with high yield.
There are also known processes for the hydrolysis of yeast cells by thermal, enzymatic or chemical treatment, permitting producing yeast extracts for alimentary or therapeutic uses.
However, these known processes, often based on acid hydrolyses, lead to preparations in the form of hydrolysates containing together the proteins and the polysaccharides of yeast, and cannot supply yeast extracts specifically enriched in glycogens.
It is known moreover to extract glycogens from marine bacteria, and to subject these latter to a thermal and chemical treatment, to a trichloroacetic acid precipitation and to consecutive precipitations with ethanol.
These extractions, carried out on a laboratory scale, have only for their object to permit the analysis of the structure and the composition of the soluble glycogens and are not applicable other than to the specific type of cells in question, and are not applicable to cells having more resistant walls.
Finally, there already exist processes for the permeabilization of the cell walls of yeast and for the hydrolysis of the intracellular glycogens in glucose, so as to measure the amount present, these processes however cannot ensure extraction of the glycogen as such, particularly in an unhydrolyzed form.
OBJECT OF THE INVENTION
The problem addressed by the present invention consists accordingly in designing a process for the production of glycogens or extracts rich in glycogens from yeast cells, which can be easily practiced industrially for the production of glycogens in large quantity, having a high yield relative to the quantity of initial material treated, permitting extracting soluble and insoluble glycogens (connected to proteins of the cell walls) and hence to obtain extracts of variable glycogen content, particularly of very high values and in the non-hydrolyzed form, and adapted as the case may be to use residual yeasts from fermentation processes.
SUMMARY OF THE INVENTION
This problem is precisely solved by the production process according to the invention, characterized in that it consists essentially in taking a given quantity of yeast cells, supplied from a specific culture or recovered as residues of a fermentation process, subjecting said yeast cells to an operation of enrichment in intracellular glycogen in the presence of a carbon source, blocking the metabolism of said yeast cells, disintegrating at least partially the membranes of said yeast cells and subjecting the intracellular substances freed, to one or more overall or selective precipitations, as the case may be after the application of a fractionation, concentration, demineralization and/or decoloration operation.
DETAILED DESCRIPTION OF THE INVENTION
The above yeast cells can consist for example of yeasts recovered from different stages of existing processes for the production of yeast or for the use of yeast, for example in the course of producing beer.
However, said yeast cells can also be obtained by specific propagation processes carried out in fermenters containing a nutrient medium permitting the growth of yeast. The propagation of the yeasts
REFERENCES:
patent: 3867255 (1975-02-01), Newell et al.
patent: 3867554 (1975-02-01), Sucher et al.
patent: 3888839 (1975-06-01), Newell et al.
patent: 5571503 (1996-11-01), Mausner
(R)Dedhia et al., "Overproduction of glycogen in E. coli blocked in the acetate pathway improves cell growth . . . ", Biotechnol. Bioeng., 1994, vol. 44, issue 1, pp. 132-139.
(S) Peterson et al., "Enhancement of carbohydrates in a methylotrophic yeast . . . ", Enzyme Microb. Technol.; (1983) 5, 5, pp. 337-341.
Engasser Jean-Marc
Ghoul Mohamed
Pauly Gilles
Pauly Marc
Laboratoires Serobiologiques (Societe Anonyme)
Naff David M.
Ware Deborah K.
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