Method for producing factor VIII:C and analogs

Details Method for producing factor VIII:C and analogs Method for producing factor VIII:C and analogs

1991-05-23

1993-03-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4351723, 4352402, 43524031, 4353201, 435948, 530383, 530381, 530380, 935 11, 935 70, 935 10, 935 60, 930100, C12P 2102, C12N 1510, C12N 516, C12N 1585

Patent

active

051983494

ABSTRACT:
An improved method for producing Factor VIII:c is disclosed. The method involves culturing mammalian cells which contain DNA encoding Factor VIII:c and which are capable of expressing Factor VIII:c. In accordance with this invention the cells are cultured in a medium containing an effective amount of a Factor VIII:c-stabilizing substance comprising (a) von Willebrand Factor (VWF), (b) a phospholipid or phospholipid mixture, or a mixture of (a) and (b).

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