Method for producing extra-chromosomal nucleic acid molecules

Details Method for producing extra-chromosomal nucleic acid molecules Method for producing extra-chromosomal nucleic acid molecules

2005-05-03

2010-11-30

Kim, Young J (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S306100, C536S025400

Reexamination Certificate

active

07842481

ABSTRACT:
The present invention relates to a method, whereby microbial cells are broken up and cellular components are separated and an apparatus suitable for carrying out the method. Preferably, the cells contain extra-chromosomal nucleic acids that are released and are further suitable for additional purification as required for any pharmaceutical or technical use.

REFERENCES:
patent: 5407426 (1995-04-01), Spears
patent: 2005/0026177 (2005-02-01), Urthaler et al.
patent: 2005/0079534 (2005-04-01), Warthen et al.
patent: WO 03102184 (2003-12-01), None
patent: WO 2004024283 (2004-03-01), None
patent: WO 2004108260 (2004-12-01), None
Theodossiou et al. Methods of enhancing the recovery of plasmid genes from neutralised cell lysate. Bioprocess Engineering (1999) 20: 147-156.

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