Method for producing ethanolamine oxidase

Details Method for producing ethanolamine oxidase Method for producing ethanolamine oxidase

1986-05-21

1988-11-29

Warden, Robert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 25, 435832, C12N 906, C12Q 126

Patent

active

047881470

ABSTRACT:
Ethanolamine in a sample can be assayed by treating the sample with ethanolamine oxidase, thereby to catalyze a reaction-consuming ethanolamine, oxygen and water, and forming glycolaldehyde, ammonia and hydrogen peroxide. The amount of consumed oxygen or the amount of generated ammonia or hydrogen peroxide is then determined, as a measure of the ethanolamine that was originally the sample. The ethanolamine can appear in the sample as such, or can be liberated simultaneously with or prior to the catalysis reaction, from an ethanolamine derivative, e.g. phosphatidyl ethanolamine by the action of phospholipase D. Ethanolamine oxidase can be produced from Bacillus sp. B-0783 FERM-P No. 5798 in a conventional culture medium, preferably by submerged aeration liquid culturation.

REFERENCES:
patent: 4135980 (1979-01-01), Ikuta et al.
patent: 4353983 (1982-10-01), Siddiqi
Taki et al, "Phospholipase D from Rat Brain" in Methods in Entymology, ed. by Lowenstein (NY, Academic Press, 1981), pp. 746-750.
Lehninger, "Biochemistry", Second Edition, NY, Worth Publishers, 1975, pp. 288-291.
deKoning, abstract of "Determination of Ethanolamine and Serine in Phospholipids" in Chemical Abstracts, vol. 65 (1966), No. 17341h.
Hayashi et al, abstract of "Reagents for Determination of Lecithins" in Chemical Abstracts, vol. 88 (1978), No. 88:132931f.
Bergmeyer, Methods of Enzymatic Analysis, vol. 1, NY, Academic Press, 1974, p. 504.
Narrod et al, "Metabolism of Ethanalamine", Journal of Biological Chemistry, vol. 239, No. 7, Jul. 1964, pp. 2189-2193.

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