Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1998-12-04
2000-08-29
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435171, 4352541, 435911, C12P 718
Patent
active
061107158
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a method for producing erythritol using microorganisms and more precisely to a method for producing erythritol using strains belonging to Trichosporonoides megachiliensis.
BACKGROUND ART
Two microorganisms, namely, Moniliella tomentosa var. pollinis CBS461.67 and Aureobasidium sp. SN-G42 FERM P-8940, are known currently to be employed practically to produce erythritol.
The former is employed, for example, in methods for producing polyols in an industrial scale by means of fermentation of saccharides (Japanese Patent Publication No. 6-30591 (30591/1994), ibid 6-30592 (30592/1994), ibid. 6-30593 (30593/1994), ibid 6-30594 (30594/1994)), and in these publications methods for producing a series of polyols including erythritol are disclosed.
On the other hand, the latter is disclosed in Japanese Patent Publications 4-11189 (11189/1992) and ibid 4-635 (635/1992) in which a novel microorganism having an erythritol producing ability and a method for producing erythritol by means of fermentation using such microorganism are described.
Meanwhile, a microorganism belonging to genus Trichosporonoides was reported by Marina A. Y. Aoki et al. of the State University of Campinus in Brazil (Biotechnology Letters, Volume 15, No.4, p. 383-388, April 1993) to be employed in conversion from sucrose and glucose to erythritol, although the species is not known. According to this report, the rates of conversion from glucose to erythritol and sucrose to erythritol were as relatively high as 43.0% and 37.4%, respectively, but the saccharide concentration for such yields was as low as 10 w/v % and the cultivation thereof takes as many days as six days, indicating a poor applicability to a production in an industrial scale.
Trichosporonoides megachiliensis employed in the present invention was reported to be a new strain by G. Douglas Inglis and Lynne Sigler of the University of Alberta in Canada (Mycologia, Volume 84, No. 4, p. 555-570, 1992). Their report contained description of morphological and physiological characteristics of a strain identified as a new strain but no description of its ability of producing erythritol.
We, the present inventors, have studied the method for producing erythritol with a use of microorganisms and finally found that Trichosporonoides megachiliensis converted saccharaides such as glucose to erythritol effectively with a high yield and completed the present invention.
DISCLOSURE OF THE INVENTION
An object of the present invention is to provide a method for producing erythritol by culturing a strain belonging to Trichosporonoides megailiensis in a medium containing saccharide at high concentration, especially at a concentration of 20-50 w/v % and recovering erythritol from culture fluid.
The present invention relates to a method for producing erythritol comprising cultivating a strain of Trichosporonoides megachiliensis in a culture medium at high saccharide concentration and then recovering the erythritol accumulated in the culture medium.
PREFERRED EMBODIMENT OF THE INVENTION
Examples of the strains belonging to Trichosporonoides megachiliensis employed in the present invention are Trichosporonoides megachiliensis CBS 190.92 strain (UAMH 6490), Trichosporonoides megachiliensis CBS 191.92 strain (UAMH 6822) as well as those strains reported by G. Douglas Inglis et al. of the University of Alberta in Canada such as Trichosporonoides megachiliensis UAMH 6820 strain, Trichosporonoides megachiliensis UAMH 6821 strain, Trichosporonoides megachiliensis UAMH 6823 strain (these strains are stored at the University of Alberta Microfungus Collection and Herbarium) etc. and the variant obtained by treating those strains by a standard mutating method, such as physical mutating methods such as ultraviolet irradiation and radioactive irradiation as well as chemical mutating methods using chemical mutating agents such as ethyl methanesulfonic acid, nitrosoguanidine, etc.
The strain is cultivated aerobically in a liquid medium containing carbon source, nitro
REFERENCES:
Japan Abstract 09154589 Published Jun. 17, 1997 Makoto Et Al "Production of Erythritol" Mitsubishi Chem Corp.
Aoki et al, "Microbial Transformation of Sucrose and Glucose to Erythritol", Biotechnology Letters, vol. 15, No. 4, pp. 383-388 (Apr. 1993).
Inglis et al, "Trichosporonoides megachiliensis", Mycologia, vol. 84, No. 4, pp. 555-570 (1992).
Chida Saburo
Ochiai Toshiro
Lilling Herbert J.
Nikken Chemicals Co., Ltd.
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