Method for producing .epsilon.-poly-L-lysine

Details Method for producing .epsilon.-poly-L-lysine Method for producing .epsilon.-poly-L-lysine

1994-02-23

1995-07-18

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

4351723, 435115, 43525235, 4352535, C12N 120, C12N 1500

Patent

active

054340608

ABSTRACT:
The present invention provides variant strains mass-producing .epsilon.PL which are obtained by variant treatment of a strain producing .epsilon.PL. For using the variant strains, the strains are cultured in a cultivated medium, .epsilon.PL is mass-produced and stored in the culture solution, and the stored .epsilon.PL is collected from the solution. For producing .epsilon.PL, a strain which produces .epsilon.PL is variant-treated, the obtained variant strain is cultivated in a culture medium to which L-lysine or L-lysine and one or more sugars are added, .epsilon.PL is mass-produced and stored in the culture solution, and the stored .epsilon.PL is collected from the solution.
The variant strains producing .epsilon.PL in large quantities are preferably the strains which have tolerance to an analogue of L-lysine of Streptomyces albulus subsp. lysinopolymerus No. 346-D strain and plasmid-amplifiable variant strains of the same bacteria.

REFERENCES:
patent: 4411991 (1983-10-01), Hirakawa et al.
Maniatis et al, Molecular Cloning . . . p. 313, 88 1982.

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