Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1984-08-27
1988-05-03
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4351723, 435189, 935 9, 935 14, C12P 2102, C12N 1500, C12N 902
Patent
active
047420049
ABSTRACT:
A method of producing enzymatically active eucaryotic superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase or analog thereof comprising maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Cu++ so that the concentration of Cu++ in the medium is greater than about about 2 ppm.
The invention also concerns methods of recovering purified enzymatically active eucaryotic superoxide dismutase or analogs from bacterial cells producing the superoxide dismutase or analog.
REFERENCES:
patent: 3579495 (1971-05-01), Huber
patent: 4340675 (1982-07-01), Johansen
patent: 4390628 (1983-06-01), Johansen
Lieman-Hurwitz et al., P.N.A.S. 79:2808-2811, 1982 (May).
Lehninger, In Biochemistry, 2nd Ed., Worth Publishers, 1975.
Hartz, J. W. et al., J. Biol. Chem. 247: 7043 (1972).
Jabusch, J. R. et al., Biochemistry 19: 2310 (1980).
Barra et al., FEBS Letters 120: 53 (1980).
McCord, J. M. et al., J. Biol. Chem. 244: 6049 (1969).
Fridovich, I., Advances in Inorganic Biochemistry, (Eichhorn and Marzilli, eds. ) (Elsevier/North Holland, New York) pp. 67-90 (1979).
Freeman, B. A. et al., Laboratory Investigation 47: 412 (1982).
Steinman, H. M., Superoxide Dismutase, (Oberly, ed.) CRC Press, Florida, pp. 11-68 (1982).
Sherman, L. et al., Proc. Natl. Acad. Sci. USA 80: 5465 (1983).
Huber, W. et al., Clinics in Rheum. Dis. 6: 465 (1980).
McCord, J. M., Can. J. Physiol. Pharma. 60: 1346 (1982).
Alvarez, J. G. et al., Biol. Reprod. 28: 1129 (1983).
Talmasoff, J. M. et al., Proc. Natl. Acad. Sci. USA 77: 2777 (1980).
Bartfeld Daniel
Hartman Jacob R.
Kanner Dov
Bio-Technology General Corp.
Mays Thomas D.
White John P.
Wiseman Thomas G.
LandOfFree
Method for producing enzymatically active eucaryotic sod in bact does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing enzymatically active eucaryotic sod in bact, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing enzymatically active eucaryotic sod in bact will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1505901