Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2005-01-04
2005-01-04
Moran, Marjorie (Department: 1631)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C536S024330, C702S019000
Reexamination Certificate
active
06838266
ABSTRACT:
DNA is produced by preparing oligomers having partial sequences selected according to a specific scheme based on a target nucleotide sequence, and performing PCR using two of single strand DNAs base-paired at their 3′ ends as primers and templates to prepare DNA, in a specific manner.
REFERENCES:
patent: 9410315 (1994-05-01), None
patent: 9916904 (1999-04-01), None
Sandhu et al. Dual Asymmetric PCR: One-Step Construction of Synthetic Genes. BioTechniques. 1992, vol. 12, No. 1, pp. 14-16.*
Chrisotomos Prodromou and Laurence H. Pearl, Recursive PCR: a novel technique for total gene synthesis, Oxford University Press, 1992, pp827-829.
Willem P.C.Stemmer, Andreas Crameri, Kim D.Ha, Thomas M.Brennan and Herbert L.Heyneker, Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides, Gene 164, 1995 pp49-53.
Horton, et al. “Engineering Hybrid Genes Without the Use of Restriction Enzymes: Gene Splicing by Overlap Extension,”Gene, vol. 77, pp. 81-88, 1989.
Sandhu, et al. “Dual Asymmetric PCR: One-Step Construction of Synthetic Genes,”BioTechniques, vol. 12, No. 1, pp. 14-16, 1992.
White, et al. “Concatemer Chain Reaction: A Tag DNA Polymerase-mediated Mechanism for Generating Long Tandemly Repetitive DNA Sequences,”Analytical Biochemistry, vol. 199, pp. 184-190, 1991.
Ye, et al. “Gene Synthesis and Expression inE. colifor Pump. A Human Matrix Metalloproteinase,”Biochemical and Biophysical Research Communicationsl, vol. 186, No. 1, pp. 143-149, 1992.
European Search Report completed Dec. 4, 2003 and issued in a related pending foreign application.
Chow Lori A.
Moran Marjorie
Nisshinbo Industries Inc.
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