Method for producing dinucleoside polyphosphate, nucleoside poly

Details Method for producing dinucleoside polyphosphate, nucleoside poly Method for producing dinucleoside polyphosphate, nucleoside poly

1993-04-15

1994-04-26

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 41, 435 88, 435 90, 435193, 435194, 435832, 435942, C12P 1938, C12P 1934, C12N 910

Patent

active

053066294

ABSTRACT:
A method for producing a dinucleoside polyphosphate, a nucleoside polyphosphate or a derivative thereof which comprises using adenosine-5'-triphosphate, polyphosphate or a derivative thereof and a sulfate as reaction substrates and forming a dinucleoside polyphosphate, nucleoside polyphosphate or derivatives thereof via a two-stage reaction through the use of two enzymes, namely, adenosine-5'-triphosphate sulfurylase and diadenosine tetraphosphate phosphorylase as catalysts. Further, another method for producing a dinucleoside polyphosphate, a nucleoside polyphosphate or a derivative thereof which comprises using adenosine-5'-triphosphate, polyphosphate or a derivative thereof and a sulfate as reaction substrate in the presence of an enzyme capable of converting adenosine-5'-diphosphate into adenosine-5'-triphosphate, and forming the dinucleoside polyphosphate, nucleoside polyphosphate or derivatives thereof via a two-stage reaction through the use of two enzymes, namely, adenosine-5'-triphosphate sulfurylase and diadenosine tetraphosphate phosphorylase as catalysts is provided. According to the present invention, highly pure dinucleoside polyphosphate, nucleoside polyphosphate or derivatives thereof can be produced at a high yield and these products can be easily isolated and purified.

REFERENCES:
patent: 4971903 (1990-11-01), Hyman
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P. F. Maness et al., J. Biol. Chem., 258, 4055 (1983).
M. J. Harrison et al., FEBS Letters, 54, 57 (1975).
O. Goerlich et al., Eur. J. Biochem., 126, 135 (1982).
A. Guranowski et al., Biochemistry, 27, 2959 (1988).

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