Method for producing dendritic cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology

Reexamination Certificate

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C435S325000, C435S354000, C435S366000, C435S386000, C435S320100, C424S093200, C424S093210

Reexamination Certificate

active

09849499

ABSTRACT:
Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified.

REFERENCES:
patent: WO 97/21802 (1997-06-01), None
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F. A. Brook and R. L. Gardner, “The origin and efficient derivation of embryonic stem cells in the mouse,”Proc. Natl. Acad. Sci. USA, 97: 5709-5712 (1997).
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Senju et al. Generation and genetic modification of dendritic cells derived from mouse embryonic stem cells. Immunogiology, BLOOD, 2003, vol. 101, No. 9, pp. 3501-3508.
Slukvin, et al. Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathway. The Journal of Immunology, 2006, 176: 2924-2932.

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