Method for producing D-alanine

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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4352551, 4352541, 435911, 435938, 435921, 435944, C12P 1306, C12N 116

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054222559

ABSTRACT:
A high concentration of DL-alanine can be supplied in a culture medium and D-alanine can be efficiently obtained with high yield for a short time by cultivating a yeast which belongs to the genus Candida, the genus Cryptococcus, the genus Hansenura or the genus Trichosporon and has an ability to assimilate L-alanine and not to assimilate substantially D-alanine in a culture medium containing substantially DL-alanine as a single carbon source and a single nitrogen source under an acidic condition. Moreover, because very little other organic by-product and organic impurity exists in the culture medium when the cultivation is completed, it becomes easy to separate and refine the D-alanine.

REFERENCES:
patent: 4783404 (1988-11-01), Aretz et al.
ATCC Catalogue of Fungi, 1987 p. 73.
ATCC Catalog of Fungi, 1987, 17th ed, pp. 123, 184, 290, 381.
Chemical Abstracts, vol. 67 1967 #9 42581d "Preparation of optically active alanine from DL-alanine by yeast" Oshima et al. Hakko to Taisha No. 15 89-94 discussion 94 (1967) (Japan).

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