Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1994-03-24
1996-06-25
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351723, 4351721, 47 58, 47DIG1, A01H 500, A01H 100, C12N 1505
Patent
active
055301916
ABSTRACT:
The present invention provides a cytoplasmic male sterility (CMS) system for plants, which is based on modification of the plastid genome. The CMS system comprises three transgenes: a "plastid male sterility" (pms) gene, regulated by a "nuclear male sterility" (rims) gene and a "restorer of male fertility" (rmf) gene. The pms gene is either an inactive gene encoding a toxic gene product or an actively expressed gene encoding an essential gene product. The runs gene is controlled by an anther-specific promoter, and encodes a plastid-directed polypeptide that regulates the pms gene, either by activating expression of the inactive toxic pms gene or by repressing expression of the essential pms gene, which causes plastid and cellular disablement or death in anther tissue, thereby preventing formation of viable pollen. The rmf gene encodes a gene product that prevents the rims gene from regulating the pms gene. Cytoplasmically male-sterile plants are obtained by crossing a parent line containing the pms gene with a parent line containing the nms gene. Male-fertile hybrid seed is obtained from crosses between the cytoplasmically male-sterile plants and a second parent line containing the rmf gene.
REFERENCES:
patent: 4918006 (1990-04-01), Ellar et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 5122457 (1992-06-01), Reim et al.
An, Plant Physiol., 81: 86-91 (1986).
Bevan, Nuc. Acids Res., 12: 8711-8721 (1984).
Brent & Ptashne, Cell, 43: 729-736 (1985).
Carey et al., J. Mol. Biol., 209: 423-432 (1989).
Carrer et al., Plant Mol. Biol., 17: 301-303 (1991).
Chen et al., Cell, 50: 1047-1055 (1987).
Cress et al., Science, 251: 87-90 (1991).
Czernylowski et al., DNA, 5: 101-103 (1986).
Deuschele et al., Proc. Natl. Acad. Sci., 86: 5400-5404 (1989).
Dubendorff & Studier, J. Mol. Biol., 219: 45-49 (1991).
Farabaugh, Nature, 274: 765-769 (1978).
Futterer & Hohn, EMBO J., 10: 3887-3896 (1990).
Gatz et al., Proc. Natl. Acad. Scie., 85: 1394-1397 (1988).
Gatz et al., Plant Journal, 2 (3): 397-404 (1992).
Goldberg et al., Plant Cell, 5: 1217-1229 (1993).
Gruissem & Tonkyn Critical Review in Plant Sciences, 12: 19-55 (1993).
Hajdukiewicz et al., Plant Molec. Biol., 0: 1-6 (1994) in print.
Hall et al., Cell, 36: 1057-1065 (1984).
Hanson et al., International Review of Cytology, 94: 213-267 (1985).
Hanson et al., Plant Physiol., 81: 86-91 (1986).
Horsch et al., Science, 227: 1229-1231 (1985).
Jefferson et al., EMBO J., 6: 3901-3907 (1987).
Juranka et al., FASEB, 3: 2583-2592 (1989).
Levings et al., Plant Molec. Biol., 19: 135-147 (1992).
Maliga et al., Phil. Trans R. Soc. Lond. B., 342: 203-208 (1993).
Mariani et al., Nature, 347: 737-741 (1990).
Mariani et al., Nature, 357: 384-387 (1992).
McLean et al., J. Bacteriol., 169: 1017-1023 (1986).
Ortiz et al., EMBO, 11: (10) 3491-3499 (1992).
Palmer et al., TIG, 6: 115-120 (1990).
Sadowski et al., Nature, 335: 563-564 (1988).
Schnell & Blobel, J. Cell. Biol., 120: 103-115 (1993).
Stern & Gruissem Cell, 51: 1145-1157 (1987).
Studier et al., Methods in Enzymol., 185: 60-89 (1990).
Sugiura, Plant Mol. Biol., 19: 149-168 (1992).
Svab et al., Proc. Natl. Acad. Sci., 90: 913-917 (1993).
Theg et al., Trends in Cell Biol., 3: 186-190 (1993).
Timmermans et al., Jour. Biotech., 14: 333-344 (1990).
Twell et al., Genes and Development, 5: 496-507 (1991).
Ueda and Hayasishi, Ann. Rev. Biochem., 54: 73-100 (1985).
van der Meer et al., Plant Cell, 4: 253-262 (1992).
van Haaren and Ow, Plant Molec. Biol., 23: 525-533 (1993).
Weising et al., Ann. Rev. Genet., 22: 421-477 (1988).
Methods in Plant Molecular Biology--A Laboratory Manual Maliga, Klessig, Cashmore, Gruissem, & Varner, eds., Cold Spring Harbor Press (1994, in press; include pages pertinent to pPZP vectors).
Wolfe, Stephen L. (1972) Biology of the Cell. Wadsworth Publishing Company, Inc. Belmont California. pp. 154-159.
Frosthoefel et al. (1992) Discordant inheritance of mitochrondrial and plastid diverse alfalfa genotypes. Journal of Heredity vol. 83(50. pp. 342-345. BIOSIS abstract BA95:27609.
Poehlman John, Milton. (1987) Breeding Field Crops. AVI Publishing Company, Inc. pp. 473-476.
Benzion Gary
Reed Janet E.
Rutgers The State University of New Jersey
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