Method for producing creatinase

Details Method for producing creatinase Method for producing creatinase

1982-04-23

1983-12-13

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, 435832, C12N 978, C12R 107

Patent

active

044205627

ABSTRACT:
Isolation method of creatinase is disclosed. The method comprises culturing a microorganism belonging to Bacillus, for example, B-0618 strain (deposition No. FERM-P 4049 at Fermentation Research Institute, Agency of Industrial Science and Technology, Japan) to obtain cells from the cultured product, obtaining sarcosine oxidase and creatinase-containing solution, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography to obtain a creatinase fraction and then collecting creatinase.

REFERENCES:
patent: 3907644 (1975-09-01), Mollering et al.
patent: 4039384 (1977-08-01), Suzuki et al.
patent: 4216292 (1980-08-01), Ikuta et al.

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