Method for producing copies of a nucleic acid using immobilized

Details Method for producing copies of a nucleic acid using immobilized Method for producing copies of a nucleic acid using immobilized

1995-11-22

1999-07-13

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 6, C12P 1934, C12Q 168

Patent

active

059225742

ABSTRACT:
Amplification of nucleic acids using oligonucleotides immobilized on solid phase supports is disclosed. Target nucleic acid strands (in a sample to be analyzed) are hybridized to the oligonucleotides and copy target strands (incorporating immobilized oligonucleotide) are produced using the target strands as templates. The target strands are denatured from the copy target strands and rehybridized to non-extended oligonucleotides. In the next step, new copy target 1 strands are synthesized from the oligonucleotides hybridized to the target strands, and simultaneously copy target 2 strands are synthesized using previously generated copy target 1 strands. Sequence of denaturing, rehybridizing, and producing copy target 1 and copy target 2 strands is repeated as often as necessary to give the desired degree of amplification.

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patent: 5066584 (1991-11-01), Gyllensten et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5219727 (1993-06-01), Wang et al.
patent: 5641658 (1997-06-01), Adams et al.
Mar. 15, 1994 Genetic Engineering News, vol. 14, No. 6, pp. 1, 14 and 19.

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