Method for producing cobalt-protein complex

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Metal containing – e.g. – chromoproteins – ferritin,...

Reexamination Certificate

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C530S350000, C424S009340

Reexamination Certificate

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10644774

ABSTRACT:
A method for obtaining a cobalt-apoferritin complex according to the present invention includes: the step a) of preparing a solution including a Co2+ion, a protein, a pH buffer agent and a Co2+associating agent; and the step b) of adding an oxidizing agent to the solution and thereby making the protein contain a fine particle including cobalt.

REFERENCES:
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patent: 6180389 (2001-01-01), Douglas et al.
patent: WO 2004/033366 (2004-04-01), None
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Yang et al., Biochem. J., vol. 338, pp. 615-618, 1999.
Matias et al., Acta Cryst. vol. F67, pp. 503-506, 2005.
Okuda, M. et al “Fabrication of Nickel and Chromium Nanoparticles using the Protein Cage of Apoferritin”, Biotechnology and Bioengineering Including: Symposium Biotechnology in Energy Production and Conservation, vol. 84, No. 2, Oct. 20, 2003, pp. 187-194, (XP-002975877).
Allen, M. et al, “Constrained Synthesis if Cobalt Nanomaterials in the 12-Subunit Protein Cage fromListeria innocua”, Inorganic Chemistry, vol. 42, No. 20, Oct. 6, 2003, pp. 6300-6305, (XP-002301238).
D'Souza, V. M. et al., “Divalent Metal Binding Properties of the Methionly Aminopeptidase fromEscherichia coli”, Biochemistry, vol. 39, No. 13, Apr. 4, 2000, pp. 3817-3826, (XP-002301239).

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