Method for producing cholesterol oxidase in the presence of a no

Details Method for producing cholesterol oxidase in the presence of a no Method for producing cholesterol oxidase in the presence of a no

1976-11-15

1978-06-06

Jones, Raymond N.

Textiles: fiber preparation

195114, C12D 1310

Patent

active

040935175

ABSTRACT:
An improved fermentation method comprises growing a cholesterol oxidase-producing microorganism in a medium comprising a carbon source, such as glycerol, yeast extract, a nonionic surfactant, an auxiliary carbon source which is also an inducer of cholesterol oxidase, and trace salts. Yields of cholesterol oxidase up to about 900 U. (international units) per liter have been obtained using the improved method of the present invention.

REFERENCES:
patent: 3776816 (1973-12-01), Terada et al.
patent: 3884764 (1975-05-01), Goodhue et al.
patent: 3909359 (1975-09-01), Goodhue et al.
Voets et al., "Microbial Degradation of Cholesterol," Chemical Abstracts, vol. 81, No. 1, p. 96 (1974) abs. No. 1140e.
Reese et al., "Surfactants as Stimulants of Enzyme Production by Microorganisms," Applied Microbiology,vol. 17, No. 2 (1969), pp. 242-245.

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