Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2007-08-07
2007-08-07
Carlson, Karen Cochrane (Department: 1656)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C536S023100, C530S412000, C530S350000, C435S069100, C435S007200, C435S069700
Reexamination Certificate
active
10992360
ABSTRACT:
We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.
REFERENCES:
patent: 5270181 (1993-12-01), McCoy et al.
patent: 5532151 (1996-07-01), Chantry et al.
patent: 2002/0142387 (2002-10-01), Seki et al.
patent: 1 143 009 (2001-10-01), None
patent: 1 176 210 (2002-01-01), None
patent: 1 354 959 (2003-10-01), None
patent: 9-107954 (1997-04-01), None
patent: WO 9213955 (1992-08-01), None
patent: WO 99/02671 (1999-01-01), None
patent: WO 02/090537 (2002-11-01), None
Liu et al. (Feb. 2001) Functional characterization of novel human ARFGAP3. FEBS Lett. vol. 490, Nos. 1-2, pp. 79-83.
Laage et al. (2001) Strategies for prokaryotic expression of eukaryotic membrane proteins. Traffic, vol. 2, No. 2, pp. 99-104.
Yoshida et al. (2000) In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity. J. Biol. Chem. vol. 275, No. 1, pp. 497-506.
Lehto et al. (1998) Release of the glycosylphosphatidylinositol-anchored enzyme ecto-5′-nucleotidase by phospholipase C: catalytic activation and modulation by the lipid bilayer. Biochem. J. vol. 332 (Pt 1), pp. 101-109.
The pET Expression system (2003) The pET Expression system, http://www.bio.davidson.edu/Course/Molbio?nolStudents/spring2003/Causey/pET.html, pp. 1-4.
Tucker et al. (1996) Purification of a rat neurotensin receptor expressed inEscherichia coli. Biochem. J. vol. 317 (Pt 3), pp. 891-899.
Grisshammer et al. (1993) Expression of a rat neurotensin receptor inEscherichia coli. Biochem. J. vol. 295 (Pt 2), pp. 571-576.
Abdulaev et al. Functionally discrete mimics of light-activated rhodopsin identified through expression of soluble cytoplasmic domains. J. Biol. Chem. vol. 275, pp. 39354-39363.
Sachev et al. (1998) Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin. Protein Expr. Purif. vol. 12, No. 1, pp. 122-132.
McIntyre et al. (1991) Procapthesins L and D are membrane-bound in acidic microsomal vesicles. J. Biol. Chem. vol. 266, No. 23, pp. 15438-15445.
Invitrogen (2002) Flexible in vitro expression with high-yield results, Expressions, vol. 9 issue 2, p. 7.
Novagen (1998) pET-23a-b(+)vectors, p. 1.
Falk et al. (1997) Cell-free synthesis and assembly of connexins into functional gap junction membrane channels.□□EMBO J. vol. 16, No. 10, pp. 2703-2716.
Rhee et al. (1996) Channel-forming activity of immunoaffinity-purified connexin32 in single phospholipid membranes. Biochemistry. vol. 35, No. 28, pp. 9212-9223.
Ohtaki et al. (1998) Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide. large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells. J. Biol. Chem. vol. 273, No. 25, pp. 15464-15473.
Mambetisaeva et al. (1997) Expression of three functional domains of connexin 32 as thioredoxin fusion proteins inEscherichia coliand generation of antibodies. Protein Expr. Purif. vol. 11, No. 1, pp. 26-34.
Peng et al. (1998) Cystic fibrosis transmembrane conductance regulator: expression and helicity of a double membrane-spanning segment. FEBS Lett. vol. 431, No. 1, pp.29-33.
Sigma-Aldrich product catalog page for Polyoxyethlene 23 lauryl ether (Brij 35).
E. Bochkareva et al., The Journal of Biological Chemistry, vol. 271, No. 36, Sep. 1996, pp. 22256-22261.
N. Nishimura et al., Journal of Fermenation and Bioengineering, vol. 80, No. 4, 1995, pp. 403-405.
T. Kigawa et al., FEBS Letters, vol. 442, 1999, pp. 15-19.
P.J. Booth et al., Biochemical Society Transactions, vol. 28, Part 3, 2000, p. A50.
Shuyun Peng et al., FEBS Letters, vol. 431, No. 1, pp. 29-33 (1998).
Goto Mie
Ishihara Goushi
Kigawa Takanori
Saeki Mihoro
Shirouzu Mikako
Birch & Stewart Kolasch & Birch, LLP
Carlson Karen Cochrane
Liu Samuel W.
Riken
LandOfFree
Method for producing by cell-free protein synthesis system... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing by cell-free protein synthesis system..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing by cell-free protein synthesis system... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3880319