Method for producing antithrombin-III, method for purifying it,

Details Method for producing antithrombin-III, method for purifying it, Method for producing antithrombin-III, method for purifying it,

1997-11-19

1999-11-23

Henley, III, Raymond

Drug, bio-affecting and body treating compositions

Extract, body fluid, or cellular material of undetermined...

Blood

514 8, A61K 3514, A61K 3816

Patent

active

059895934

ABSTRACT:
A method for producing antithrombin-III from an antithrombin-III-containing aqueous solution, comprising at least one of the following steps (a) and (b): (a) heating the antithrombin-III-containing aqueous solution in the presence of a stabilizing agent so that 85% or more of the activity of antithrombin-III before the heating is maintained after the heating, and that the ratio of an antithrombin-III monomer after the heating is maintained at 95% or more; and (b) treating the antithrombin-III-containing aqueous solution with a metal chelate resin and recovering a purified antithrombin-III.

REFERENCES:
patent: 3842061 (1974-10-01), Anderson et al.
patent: 4340589 (1982-07-01), Uemura et al.
J. Porath et al. "Metal Chelate Affinity Chromatography, a New Approach to Protein Fractionation" Nature, vol. 258, Dec. 18, 1975, p. 598/599 XP002059246.
Newton et al. J. Biol. Chem., 269 (43), 26739-45 (Abstract), 1944.

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