Method for producing an L-amino acid using a bacterium with...

Details Method for producing an L-amino acid using a bacterium with... Method for producing an L-amino acid using a bacterium with...

2005-09-12

2009-10-20

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C536S023100

Reexamination Certificate

active

07604979

ABSTRACT:
A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genusEscherichiawith an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.

REFERENCES:
patent: 5378616 (1995-01-01), Tujimoto et al.
patent: 5573945 (1996-11-01), Ono et al.
patent: 5908768 (1999-06-01), Ono et al.
patent: 6960455 (2005-11-01), Livshits et al.
patent: 7332309 (2008-02-01), Rieping
patent: 2003/0148473 (2003-08-01), Livshits et al.
patent: 2004/0229320 (2004-11-01), Stoynova et al.
patent: 2005/0191684 (2005-09-01), Zimenkov et al.
patent: WO 98/07846 (1998-02-01), None
Official Communication from European Patent App. No. 04720229.6 (Dec. 18, 2007).
Datsenko, et al., “One-step inactivation of chromosomal genes inEscherichia coliK-12 using PCR products,” PNAS 2000;97(12):6640-6645.
Horwitz, M. S. Z., et al., “Promoters selected from random DNA sequences,” Proc. Natl. Acad. Sci. USA 1986;83:7405-7409.
Solem, C., et al., “Modulation of Gene Expression Made Easy,” Appl. Environment. Microbiol. 2002;68(5):2397-2403.
International Search Report for PCT Application No. PCT/JP2004/003377 (Sep. 10, 2004).
Written Opinion of the International Searching Authority for PCT Application No. PCT/JP2004/003377 (Sep. 29, 2004).

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