Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-07
1998-04-14
Weber, Jon P.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435814, 435815, C12N 950
Patent
active
057390258
ABSTRACT:
A method is provided for preparing an asparaginyl endoproteinase from the seeds of soybean, ginkgo and rice which have been collected between an early growing stage and ripening. The method comprises the steps of dialyzing an extract of the seeds against an acidic buffer of pH 4.0 to 6.0, ammonium sulfate precipitation, hydrophobic chromatography and gel filtration. The resulting asparaginyl endoproteinase cleaves glycinin between the C-terminal amino acid residue of the acidic subunit region, Asn, and the N-terminal amino acid residue of the basic subunit region, Gly or Asn.
REFERENCES:
Shutov et al. (1982) Biokhimiya, 47(5), "Purification and Partial Characterization of Protease B from Germinating Vetch Seeds", pp. 814-822.
Matsushita et al. (1991) Tanpankushitsu Kakusan Koso (Prot. Nucl. Acid Enzym.), 36(4), "Asparaginylendopeptidase", pp. 730-736 (English translation is provided. Japanese is in parent application).
Pesce et al. (1967) J. Biol. Chem., 242(9), "The Comparative Enzymology of Lactic Dehydrogenase", pp. 2151-2167.
Shin-ichi Ishii et al, "An Asparaginyl Endopeptidase Purified from Jackbean Seeds", vol. 9, pp. 294-295, (1990), J. Protein Chem.
Ikuko Hara Nishimura et al, "A Unique Vacuolar Processing Enzyme Responsible for Conversion of Several Proprotein Precursors into the Mature Forms", vol. 294, No. 1, 2, pp. 89-93, (1991) Febs Letters.
M. Scott et al, "A Protease Responsible for Post-Translational Cleavage of a Conserved Asn-Gly Linkage in Glycinin, the Major Seed Storage Protein of Soybean", vol. 89, pp. 658-662, (1992) Proc. Natl. Acad. Sci. USA.
Director of National Food Research Institute, Ministry of Agricu
Weber Jon P.
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