Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector
Patent
1993-04-01
1997-06-24
Eisenschenk, Frank C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
4241931, 5303881, 5303889, 5303891, 5303898, C07K 1644, A61K 39385
Patent
active
056414889
ABSTRACT:
The invention relates to a method for producing antibodies of desired specificity. In particular, the method involves immunizing a subject animal with the chosen antigen following prior stimulation of the non-human animal's B cells. The prior stimulation may be accomplished, e.g., by immunization with a first antigen which is different from the second antigen. In a particularly preferred embodiment, the animal is a transgenic animal containing a human immunoglobulin gene, such as a transgenic mouse.
REFERENCES:
Ellenberger et al., "Recruiting Memory B Cells With Changed Antigenic Specificity" , J. Immunol. 151(10: 5272-5281 (Nov. 15, 1993).
Liu et al., "Sequencing heavy-and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification", Proc. Natl. Acad. Sci. 89: 7610-7614 (Aug. 1992).
Strong et al., "Three Dimensional Structure of Murine Anti-p-azophenylarsonate Fab 36-71. 1. X-ray Crystallography, Site-Directed mutagenesis, and Modeling of the Complex with Hapten", Biochem. 30(15): 3739-3748 (1990).
Parhami-Seren et al., "Clustered H Chain Somatic Mutations Shared by Anti-p-Azophenylarsonate Antibodies Confer Enhanced Affinity And Ablate The Cross Reactive Idiotype", J. Immunol. 245(7): 2340-2346 (Oct. 1990).
Wysocki et al., "Parallel Evolution of Antibody Variable Regions by Somatic Processes: Consecutive Shared Somatic Alterations in V.sub.H Genes Expressed by Independently Generated Hybridoma Apparently Acquired by Point Mutation and Selection Rather than by Gene Conversation", J. Exp. Med. 172: 315-323 (1990).
Fish et al., "Molecular Analysis of Original Antigenic Sin:," J. Exp. Med. 170:1191-1209 (Oct. 1989).
Wysocki et al., "Single Germline V.sub.H and V.sub.K Genes Encode Predominanting Anti-body Variable Regions Elicited in Strain A Mice By Immunization with p-Azopehnylarsonate", J. Exp. Med. 166: 1-11 (Jul. 1987).
Manser et al., "Evolution of Antibody Variable Region Structure During the Immune Response", Immunol. Rev. 96: 141-162 (1987).
Wysocki et al., "Somatic evolution of variable region structures during an immune response", Proc. Natl. Acad. Sci. USA 83: 1847-1851 (Mar. 1986).
Wysocki et al., "Combination Diversity Within Variable Regions Bearing The Predominant Anti-p-Azophenylarsonate Idiotype of Strain A Mice", J. Immunol. 134(4):2740-2747 (Apr. 1985).
Manser et al., "Isolation of hybridomas expressing a specific heavy chain variable region gene segment by using a screening technique that detects mRNA sequences in whole cell lysates", Immunol. 81: 2470-2474 (Apr. 1984).
Rothstein. "Affinity Analysis of Idiotype-Positive and Idiotype Negative Ars-Binding Hybridoma, Proteins & Ars-Immune Sera", Mol. Immunol. 20(2): 161-168 (1983).
Siekevitz, "The Genetic basis of antibody production; a single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse", Eur. J. Immunol. 13: 123-132 (1983).
Woodland et al., "Idiotype-specific T Helper cells are required to induce idiotype positive B memory cells to secrete antibody", Eur. J. Immunol. 8: 600-606 (1978).
Vanden Berg et al., Immunology, 1980, 40:673.
Goding, Monoclonal Antibodies:Principles & Practice, 1986, pp. 61-62.
Wilhelm et al., Crit. Rev. Eucary Gene Express., 1992, 2(2)111-135.
Eisenschenk Frank C.
National Jewish Center for Immunology and Respiratory Medicine
LandOfFree
Method for producing an antibody to a chosen antigen does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing an antibody to a chosen antigen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing an antibody to a chosen antigen will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-147708