Method for producing active human alanine aminotransferase

Details Method for producing active human alanine aminotransferase Method for producing active human alanine aminotransferase

1997-09-30

1999-09-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352523, 43525233, 4353201, 536 231, 536 232, 536 235, C12N 910, C07H 2104

Patent

active

059522119

ABSTRACT:
The invention relates to a mutated human ALT (alanine aminotransferase) gene improving the DNA sequence encoding the N-terminal region of human ALT, without causing amino acid substitution, while, at the same time, adding restriction endonuclease sites to the upstream and downstream sites of the gene. By cultivation of E. coli transformant carrying a recombinant plasmid, which was constructed by ligation of the mutated human ALT gene into a vector, an active human ALT can be produced efficiently.

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