Method for producing a transgenic plant with the aid of...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C800S278000, C800S298000, C800S294000, C800S317400, C435S430100, C435S468000, C435S430000

Reexamination Certificate

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07947871

ABSTRACT:
The invention relates to an improved method foragrobacteriumtransformation and regeneration of plants. The inventive method is characterized in that it consists in sequentially preparing, inoculating and co-cultivating explants. The preparation of each subsequent lot of plants is carried out after a time interval for transforming plant cells and forming an induced resistance with respect to abiotic and biotic stresses in a leaf discs, thereby making it possible to reduce a necrosis degree and the number of somaclonal variations of the transgenic plants.

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