Method for producing a thermostable lipoprotein lipase from stre

Details Method for producing a thermostable lipoprotein lipase from stre Method for producing a thermostable lipoprotein lipase from stre

1992-09-03

1993-08-03

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435186, 4352535, 435906, C12N 900, C12N 920, C12Q 144

Patent

active

052328465

ABSTRACT:
The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.

REFERENCES:
patent: 4283494 (1981-08-01), Kokusho et al.
Mamara et al. (1968) Gifo Yakka Daigaku Kiyo, 18, 47-53, in Chem. Abst. 72 (1970) 34, Abst #62942.
Tadahiko (1977) Jpn. Kokai Tokkyo Koho, 78,104,792, in Chem Abst. 90 (1979) 436, Abst. #101,958.
Okawa et al. (1975) J. Biochem, 78, 363-372.
Okawa et al, (1975, J. Biochem., 78, 537-545.
Yamaguchi et al. (1973) Agric. Biol. Chem., 37(7), 1667-1672.
Aisaka et al. (1980) Agric. Biol. Chem., 44, 799-805.
Gowland et al, FEMS Microbiology Letters, 48:339-343 (1987).

Affiliated with

Also associated with

Rating

Method for producing a thermostable lipoprotein lipase from stre does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing a thermostable lipoprotein lipase from stre, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a thermostable lipoprotein lipase from stre will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2269872