Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2007-08-28
2007-08-28
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
Reexamination Certificate
active
10851383
ABSTRACT:
Disclosed herein is a method for synthesizing a desired nucleic acid sequence. The method comprises dividing the desired sequence into a plurality of partially overlapping segments; optimizing the melting temperatures of the overlapping regions of each segment to disfavor hybridization to the overlapping segments which are non-adjacent in the desired sequence; allowing the overlapping regions of single stranded segments which are adjacent to one another in the desired sequence to hybridize to one another under conditions which disfavor hybridization of non-adjacent segments; and filling in, ligating, or repairing the gaps between the overlapping regions, thereby forming a double-stranded DNA with the desired sequence. Also disclosed is a method for preventing errors in the synthesis of the nucleic acid sequence.
REFERENCES:
patent: 6472184 (2002-10-01), Hegemann
patent: 6586211 (2003-07-01), Stahler et al.
patent: 6673552 (2004-01-01), Frey
patent: 2003/0068633 (2003-04-01), Beishaw et al.
patent: 2003/0162265 (2003-08-01), Evans et al.
patent: 2003/0215837 (2003-11-01), Frey et al.
patent: 44 23 183 (1994-01-01), None
patent: 102 32 507 (2002-07-01), None
patent: WO94/12632 (1994-09-01), None
patent: WO99/11820 (1999-11-01), None
Jayaraman, Krishna and Shah, Janack,Nucleic Acids Research, Symposium Series No. 20 1998 (107-109), “Synthetic gene constructions using improved gene synthesis procedures.”
Horton, Robert M., Hunt Henry D., Ho, Steffan N., Pullen, Jeffrey K. and Pease, Larry R.,Department of Immunology and Department of Biochemistry and Molecular Biology, Mayo Clinic, Dec. 19, 1988, (61-68), “Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.”
Prodromou, Chrisostomos and Pear, Laurence H.,Protein Engineering, vol. 5, No. 8, (827-829), 1992, “Recursive PCR: a novel technique for total gene synthesis.”
Jayaraman, Krishna and Puccini, Christopher J.,Bio Techniques, vol. 12, No. 3 1992. “A PCR-Mediated Gene Synthesis Strategy Involving the Assembly of Oligonucleotides Representing Only One of the Strands.”
Dillon, Patrick J. and Rosen, Craig A.,Methods of Molecular Biology, PCR Protocols, Current Methods and Applications, vol. 15, Chapter 26, (263-269), 1993, “Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes.”
Bambot, Shabbir B. and Russell, Alan J.,PCR Methods and Applications, vol. 2, No. 3, Feb. 1993, “Efficient Total Gene Synthesis of 1.35-kb Hybrid a-lytic Protease Gene Using the Polymearse Chain Reaction.”
Hiltunen, Timo, Raja-Honkala, Maria, Nikkari, Tapio and Yla-Herttuala, Seppo,University of Tampere, Tampere, Finland, Accepted Mar. 10, 1994, “Assembly of Humanized Antibody Genes from Synthetic Oligonucleotides Using a Single-Round PCR.”
Stemmer, Willem P.C., Crameri, Andreas, Ha, Kim D., Brennan, Thomas M. and Heyneker, Herbert L.,Affymax Research Institute and PhotoGene Laboratories, Inc., Jul. 24, 1995 (49-53), “Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.”
Kobayashi, Norihiro, Soderlind, Eskil and Borrebaeck, Carl A.K.,BioTechniques, vol. 23, No. 3 (1997), “Analysis of Assembly of Synthetic Antibody Fragments: Expression of Functional scFv with Predefined Specificity.”
Casimoro, Danilo R., Wright, Peter E. and Dyson, Jane H.,Structure, vol. 5, No. 11 (1997), “PCR-based gene synthesis and protein NMR spectroscopy.”
SantaLucia, John Jr.,Proc. National Academy of Science USA, vol. 95, (1460-1465), Feb. 1998, “A unified view of polymer, dumbbell, and oligonucleotid DNA nearest-neighbor therodynamics.”
Zucker, M., Mathews, D.H. and Turner, D.H.,RNA Biochemistry and Biotechnology11-43, 1999, “Algorithms and thermodynamics for RNA Secondary Structure Prediction: A Practical Guide.”
Mathews, David H., Sabina, Jeffrey, Zuker, Michael and Turner, Douglas H.,Journal of Molecular Biology, vol. 288, (911-940), 1999, “Expanded Sequence Dependence of Thermodynamic Parameters Improves Prediction of RNA Secondary Structure.”
Martinez-Whithers, Chrislaine, Carpenter, Elisabeth P., Hackett, Fiona, Ely, Barry, Sajid, Mohammed, Grainger, Muni and Blackman, Michael J.,Protein Engineering, vol. 12, No. 12 (1113-1120), 1999, “PCR-based Gene Synthesis as an Efficient Approach for Expression of the A+ T-rich Malaria Genome.”
Lathrop, Richard H., Sazhin, Anton and Sun, Ye,Genome Informaticsvol. 12 (73-82), 2001, “A Multi-Queue Branch-and-Bound Algorithm for Anytime Optimal Search with Biological Applications.”
Hoover, David M. and Lubkowkski, Jacek,Nucleic Acids Research, vol. 30, No. 10, 2002, “DNAWorks: An Automated Method for Designing Oligonucleotides for PCR-based Gene Synthesis.”
Cello, Jeronimo, Paul, Aniko V., and Wimmer, Eckard, www. sciencemag.org, vol. 297, Aug. 9, 2002, “Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template.”
Smith, Hamilton O., Hutchison, Clyde A. III, Pfannkoch, Cynthia and Venter, J. Craig.PNAS, vol. 100, No. 26,k Dec. 23, 2003, “Generating a Synthetic Genome by Whole Genome Assembly: oX174 Bacteriophage from Synthetic Oligonucleotides.”
International Search Report in PCT Application No. PCT/US2004/016120; Dated Nov. 24, 2004; in 13 pages.
Hatfield G. Wesley
Lathrop Richard H.
Fredman Jeffrey
Knobbe Martens Olson & Bear LLP
Pande Suchira
The Regents of the University of California
LandOfFree
Method for producing a synthetic gene or other DNA sequence does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing a synthetic gene or other DNA sequence, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a synthetic gene or other DNA sequence will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3876628