Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1995-06-07
1999-09-21
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530380, 514 6, C07K 14805
Patent
active
059555815
ABSTRACT:
A method for producing a stable polymerized hemoglobin blood-substitute from blood. The method of this invention includes mixing blood with an anticoagulant to form a blood solution, washing the red blood cells in the blood solution and then separating the washed red blood cells from the white blood cells. This method also includes disrupting the red blood cells to release hemoglobin and form a hemoglobin solution, which is then treated by high performance liquid chromatography to form a hemoglobin eluate. The hemoglobin eluate is then deoxygenated, contacted with a first sulfhydryl compound to form an oxidation-stabilized deoxygenated hemoglobin solution, and mixed with a cross-linking agent to form a polymerization reaction mixture, which is then polymerized. The polymerized hemoglobin solution is then diafiltered with a physiologic solution and with a sulfhydryl compound, whereby the polymerized hemoglobin solution is made physiologically acceptable, and whereby the sulfhydryl compound scavenges oxygen, to form a stable polymerized hemoglobin blood-substitute.
REFERENCES:
patent: Re34271 (1993-06-01), Walder
patent: 3864478 (1975-02-01), Bonhard
patent: 3925344 (1975-12-01), Mazur
patent: 3959128 (1976-05-01), Harris
patent: 3991181 (1976-11-01), Doczi
patent: 4001200 (1977-01-01), Bonsen et al.
patent: 4001401 (1977-01-01), Bonsen et al.
patent: 4053590 (1977-10-01), Bonsen et al.
patent: 4059512 (1977-11-01), Harris
patent: 4061736 (1977-12-01), Morris et al.
patent: 4064118 (1977-12-01), Wong
patent: 4136093 (1979-01-01), Bonhard et al.
patent: 4314997 (1982-02-01), Shanbrom
patent: 4336248 (1982-06-01), Bonhard et al.
patent: 4376059 (1983-03-01), Davis et al.
patent: 4377512 (1983-03-01), Ajisaka et al.
patent: 4381239 (1983-04-01), Chibata et al.
patent: 4401652 (1983-08-01), Simmonds et al.
patent: 4412989 (1983-11-01), Iwashita et al.
patent: 4439357 (1984-03-01), Bonhard et al.
patent: 4473494 (1984-09-01), Tye
patent: 4473496 (1984-09-01), Scannon
patent: 4473647 (1984-09-01), Carpenter et al.
patent: 4526715 (1985-07-01), Kothe et al.
patent: 4529719 (1985-07-01), Tye
patent: 4533634 (1985-08-01), Maldonado et al.
patent: 4584130 (1986-04-01), Bucci et al.
patent: 4598064 (1986-07-01), Walder
patent: 4670417 (1987-06-01), Iwasaki et al.
patent: 4738952 (1988-04-01), Ecanow et al.
patent: 4780210 (1988-10-01), Hsia
patent: 4826811 (1989-05-01), Sehgal et al.
patent: 4857636 (1989-08-01), Hsia
patent: 4900780 (1990-02-01), Cerny
patent: 4920194 (1990-04-01), Feller et al.
patent: 4925574 (1990-05-01), Hsia
patent: 5051353 (1991-09-01), Stratton et al.
patent: 5084558 (1992-01-01), Rausch et al.
patent: 5114932 (1992-05-01), Runge
patent: 5128452 (1992-07-01), Hai et al.
patent: 5189146 (1993-02-01), Hsia
patent: 5194590 (1993-03-01), Sehgal et al.
patent: 5234903 (1993-08-01), Nho et al.
patent: 5250665 (1993-10-01), Kluger et al.
patent: 5264555 (1993-11-01), Shorr et al.
patent: 5296465 (1994-03-01), Rausch et al.
patent: 5296466 (1994-03-01), Kilbourn et al.
patent: 5334706 (1994-08-01), Przybelski
patent: 5386014 (1995-01-01), Nho et al.
Feola et al., "Complement Activation and the Toxicity of Stroma-Free Hemoglobin Solutions in Primates", Circ. Shock vol. 25, pp. 275-290. 1988,
Feola, M., et al., "Complement Activation and the Toxicity of Stroma-Free Hemoglobin Solutions in Primates, " Circulatory Shock, 25: 275-290 (1988).
Feola, M., et al., "Toxicity of Polymerized Hemoglobin Solutions, " Surgery, Gynecology& Obstetrics, 166 : 211-222 (Mar. 1988).
Feola, M., et al., "Development of a Bovine Stroma-Free Hemoglobin Solution as a Blood Substitute," Surgery, Gynecology& Obstetrics, 157 : 399-408 (Nov. 1983).
Feola, M., et al., "Biocompatibility of Stroma-Free Hemoglobin Solutions." Proceedings of The 39th Annual Conference on Engineering in Medicine and Biology, Baltimore, MD (Sep. 1986).
Gawryl Maria S.
Houtchens Robert A.
Laccetti Anthony J.
Light William R.
Rausch Carl W.
Biopure Corporation
Gupta Anish
Tsang Cecilia J.
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