Method for producing a recombinant mammal in vivo

Details Method for producing a recombinant mammal in vivo Method for producing a recombinant mammal in vivo

1989-01-27

1993-06-01

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

800 2, 800DIG2, 435948, 935 57, C12N 1500

Patent

active

052159043

ABSTRACT:
A method for producing a recombinant mammal in vivo is disclosed. The mammal has a mammary gland, with mammary epithelial cells lining a duct in that gland One increases the rate of mitosis of the mammary epithelial cells (e.g. by using perphenazine or a mitogen), and one then exposes the mammary epithelial cells in vivo in the gland to a recombinant vector (e.g. a retrovirus vector). The mammary epithelial cells can be pretreated (or simultaneously treated) with polybrene to facilitate infection by virus vectors, and the vector can have a signal sequence which causes foreign proteinaceous material to be secreted into the milk produced by the mammary gland. Also disclosed are recombinant non-human mammals produced by such methods and novel milk products that they produce.

REFERENCES:
patent: 4650764 (1987-03-01), Temin et al.
patent: 4736866 (1988-04-01), Leder et al.
patent: 4873316 (1989-10-01), Meade et al.
Edwards et al., Oncogene 2: 407-412 (1988).
Zwiebel et al., Proc. Am. Assoc. Cancer Res. Annu. Meeting vol. 31(0): 212 (1990).
Simons et al., Nature 328: 530-532 (1987).
D. Turner et al., 328 Nature 131-136 (1987).
J. Price et al., 84 P.N.A.S. USA 156-160 (1987).
J. Wilson et al., 85 P.N.A.S. USA 4421-4425 (1988).
J. Wilson et al., 85 P.N.A.S. USA 3014-3018 (1988).
J. Dougherty et al., 84 P.N.A.S. USA 1197-1201 (1987).
A. Miller et al., 12 Som. Cell Mole. Gen. 175-183 (1986).
M. Eglitis et al., 6 BioTech. 608-614 (1988).
M. Bender et al., 61 J. Virol. 1639-1646 (1987).
D. Markowitz et al., 62 J. Virol. 1120-1124 (1988).
R. Cone et al., 8 Mol. Cell. Biol. 1036-1044 (1988).
M. Ben-David, 83 Endocrinology 1217-1223 (1968).
K. Gordon et al., 5 Bio/Technology 1183-1187 (1987).
A. Dannenberg et al., Methods For Studying Mononuclear Phagocytes, pp. 375-396 (N.Y. Acad. Press) (1981).

Affiliated with

Also associated with

Rating

Method for producing a recombinant mammal in vivo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing a recombinant mammal in vivo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a recombinant mammal in vivo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1813939