Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1987-04-16
1989-11-07
Martinell, James
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
435 68, 4351721, 4351723, 435320, 935 32, 935 57, C12N 700, C12N 2100, C12N 1500
Patent
active
048792365
ABSTRACT:
A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.
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Smith Gale E.
Summers Max D.
Martinell James
The Texas A&M University System
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