Method for producing a peptidase

Details Method for producing a peptidase Method for producing a peptidase

1978-03-24

1980-10-14

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435225, 435913, 435918, C12N 948

Patent

active

042282411

ABSTRACT:
A method is provided for producing a peptidase capable of substantially completely hydrolyzing protein into its constituent amino acids which comprises culturing a strain of filamentous fungus, belonging to one of the species Aspergillus oryzae and Aspergillus soyae and characterized in that said strain is capable of producing said peptidase, in a nutrient culture medium containing at least one substrate selected from the group consisting of a fatty acid having 14, 16, 18 or 20 carbon atoms and a derivative of said fatty acid, and recovering said peptidase from said culture medium. It is particularly effective to use a combination of a sugar ester of a fatty acid and a vegetable oil as substrates in a liquid culture medium.

REFERENCES:
patent: 2888385 (1959-05-01), Grandel
patent: 3361643 (1968-01-01), Fukushima et al.
patent: 3645850 (1972-02-01), Ichishima et al.
patent: 3947323 (1976-03-01), Young
patent: 3957581 (1976-05-01), Tobe et al.

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