Method for producing a highly enriched population of osteoclast

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435325, 435366, 424 937, 424549, C12N 500

Patent

active

057190585

ABSTRACT:
Methods of obtaining enriched populations of osteoclast precursor cells which can be released from tissue culture dishes and used for biochemical studies are described. Osteoblastic cells and bone marrow cells are co-cultured. Next a .alpha..sub.v .beta..sub.3 receptor ligand, such as echistatin is used for cell detachment. The result is an 75-95% pure enriched population of tartrate resistant acid phosphatase (TRAP.sup.+) cells, in high yields (2-3.times.10.sup.6 cells per experiment). These cells are mostly mononucleated and based on their characteristics are considered to be pre-fusion osteoclasts (pOC cells). The precursor osteoclasts can be reseeded onto osteoblasts to obtain an enriched population of mature, multinucleated osteoclast cells.

REFERENCES:
patent: 5073114 (1991-12-01), Detsch
patent: 5168050 (1992-12-01), Hammonds, Jr. et al.
patent: 5169837 (1992-12-01), Largarde et al.
patent: 5258494 (1993-11-01), Oppermann et al.
patent: 5324819 (1994-06-01), Oppermann et al.
patent: 5399493 (1995-03-01), Emerson et al.
patent: 5403825 (1995-04-01), Lagarde et al.
patent: 5405772 (1995-04-01), Ponting
patent: 5437994 (1995-08-01), Emerson et al.
patent: 5486359 (1996-01-01), Caplan et al.
Wesolowski, G. et al., Experimental Cell Research, vol. 219(2), pp. 679-686, Aug. 1995.
Takahashi, N. et al., J. of Bone and Mineral Research, vol. 6(9), pp. 977-985, 1991.
Cirasole, C. et al., Ital. J. Mineral Electrolyte Metab., vol. 8(4), pp. 153-165, Dec. 1994.
Osdoby, P. et al., J. of Experimental Zoology, vol. 224, pp. 331-344, 1982.
Shiina-Ishimi, Y. et al., Biochem. & Biophys. Research Comm., vol. 134(1), pp. 400-406, Jan. 1986.
Manolagas, S.C. et al., "The Role of Vitamin D in Bone Marrow Cell Differentiation" from Vitamin D (ed. A.W. Norman et al.), pp. 675-683, 1994.
Zaidi, M. et al., Biol. Rev., vol. 68(2), pp. 197-264, 1993.
Suda, T. et al., Folia Endocrinol. vol. 67(1), pp. 144-155, 1991.
Suda, T. et al., J. Jpn. Orthop. Assoc., vol. 65, pp. 261-270, 1991.
Scheven, B.A.A. et al., Bone & Mineral, vol. 14, pp. 221-235, 1991.
Manolagas, S.C. et al., Seminars in Nephrology, vol. 14(2), pp. 129-143, Mar. 1994.
Butler, W.T., Conn. Tissue Research, vol. 23(2-3), pp. 123-136, 1989.
Abe, E. et al., J. of Bone & Mineral Research, vol. 3(6), pp. 635-645, Dec. 1988.
Van de Wijngaert, F.P. et al., Bone & Mineral, vol. 3, pp. 97-110, 1987.
Chowdhury, M.H. et al., Calcif. Tissue Int., vol. 49(4), pp. 275-279, Oct. 1991.
Hagenaars, C.E. et al., Calcif. Tissue Int., vol. 54(20, pp. 170-174, 1994.
Hentunen, T.A. et al., Biochem. & Biophys. Research Comm., vol. 209(2), pp. 433-443, Apr. 1995.
Flanagan, A.M. et al., Int. J. Exp. Path., vol. 73(3), pp. 387-401, 1992.
Takahashi, N. et al., "Endocrinology," vol. 122(4), pp. 1373-1382, 1988.
Suda, T. et al., "Bone," vol. 17(2) (supplement), pp. 87S-91S, Aug. 1995.
Manolagas, S.C. et al., "New England Journal of Med.," vol. 332(5), pp. 305-311, 1995.
Kotake, S. et al., "J. of Bone and Mineral Res.," vol. 11(1), pp. 88-95, 1996.
Kurihara, N. et al., "Endocrinology," vol. 126(5), pp. 2733-2741, 1990.
Shioi, A. et al. Calcif Tissue Intl 55 (1994) 387-394.
Gattei, V. et al. J. Cell Biol 116 (1992) 437-447 #2 Jan.
Grano, M. et al. Exp Cell Res. 212 (1994) 209-218.
Billecocq, A. et al. Proc Natl Acad Sci USA 87 (1990) 6470-6474 Aug.
Chambers, T. J. et al. Proc Natl Acad Sci USA 90 (1993) 5578-5582 Jun.
Takahashi, N. et al. Endocrinology 123 (1988) 2600-2602 #5.
Yoneda, T. et al. Endocrinology 129 (1991) 683-689 #2.
Akatsu, T. et al. J. Bone Min. Res. 7 (1992) 1297-1306 #11.
Oursler, M. J. et al. J. Bone Min. Res. 6(1991) 375-385 #4.
Grano, M. et al. J. Bone Min. Res. 9(1994) 1013-1020 #7.
Prallet, B. et al. J. Bone Min. Res. 7 (1992) 405-414 #4.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for producing a highly enriched population of osteoclast does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing a highly enriched population of osteoclast , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a highly enriched population of osteoclast will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1783469

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.