Method for producing a Gag-Env fusion protein

Details Method for producing a Gag-Env fusion protein Method for producing a Gag-Env fusion protein

1995-06-07

1998-11-10

Smith, Lynette F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, C12N 1500, C12N 1509, C12N 1563, C12N 1570

Patent

active

058342672

ABSTRACT:
Disclosed is a substantially pure HIV antigen comprising a Gag-Env fusion otein consisting of a Gag peptide fused at its C-terminus to an Env peptide, wherein the Gag peptide comprises a contiguous sequence of at least ten amino acids of the amino acid sequence represented by Gag (308-437) and the Env peptide comprises a contiguous sequence of at least a part of the amino acid sequence represented by Env (512-699), the part containing at least one epitope which is reactive to an HIV antibody. The gag-env fusion DNA corresponding to the HIV antigen of the present invention allows the production of the desired high antigenicity HIV antigen in high yield. Therefore, the HIV antigen of the present invention can be advantageously used as an active component for a diagnostic reagent, a vaccine, an antibody preparation and a therapeutic reagent for AIDS. Also disclosed is a substantially pure HIV antigen comprising a Gag protein SEQ ID No.:1 coded for by the entire gag gene.

REFERENCES:
patent: 4925784 (1990-05-01), Crowl et al.
Ellinger et al., Virology (1991), 180 (2) :811-813.
Shoeman et al. (1987). Analytical Biochemistry 161:370-379.
Siitari et al. (1990) Journal of Clinical Microbiology 28 (9) :2022-2029.

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