Method for producing a biologically active recombinant cysteine-

Details Method for producing a biologically active recombinant cysteine- Method for producing a biologically active recombinant cysteine-

1997-04-07

1999-01-19

Kemmerer, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, C12P 2102

Patent

active

058612840

ABSTRACT:
A cysteine-free peptide is produced by producing a fused protein comprising a protein having cysteine at its N-terminal and a cysteine-free peptide ligated to the N-terminal and subsequently subjecting the fused protein to a reaction for cleaving the peptide linkage.

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Jambon et al. 1988 PNAS vol. 85 (24): pp. 9426-9430.
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Nagai, et al. Methods in Enzymology, vol. 153, p. 46 (1987).

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