Method for producing a 3-D microscope flow-through cell

Details Method for producing a 3-D microscope flow-through cell Method for producing a 3-D microscope flow-through cell

2007-10-09

2007-10-09

Kim, Paul D. (Department: 3729)

Metal working

Method of mechanical manufacture

Fluid pattern dispersing device making, e.g., ink jet

C029S592100, C029S890100, C156S349000, C156S358000, C156S359000, C204S409000, C204S412000, C204S415000, C422S068100, C422S080000, C422S105000, C422S211000, C435S288500, C438S689000

Reexamination Certificate

active

10868085

ABSTRACT:
The invention relates to a method of producing a 3-D microscope flow-through cell, consisting of an upper and a lower substrate between which is located a flow channel, an electrode structure penetrating the flow channel and connected with external contacts and with through-connections at the ends of the flow channel for the connection of fluid inlets and outlets. The invention provides a method of producing 3-D microscope micro flow-through cells that are suitable for the reversible assembly of microscope flow-through cells for the μm-volume range. According to the invention, this is obtained in that a base substrate is first provided with access holes and a flow channel, the flow channel being made of a sandwich of a material non-elastic inside and elastic outside, in that the flow channel for the purpose of a fluid-tight channel closure, is pressed against a second cover glass in order to provide a reversibly sealable flow channel.

REFERENCES:
patent: 5520787 (1996-05-01), Hanagan et al.
patent: 6557582 (2003-05-01), Krog
patent: 7114541 (2006-10-01), Howitz et al.
patent: WO 02/21115 (2001-03-01), None
“Fabrication and evaluation of amperometric sensors for micro flow cell using 3-D comb electrodes”; Honda, N.; Emi, K.; Katagiri, T.; Kawakami, S.; Irita, T.; Shoji, S.; Sato, H.; Homma, T.; Osaka, T.; Microprocesses and Nanotechnology Conference, 2002; Nov. 6-8, 2002; pp. 290-291.
Warner Instruments—A Harvard Apparatus Company, “Specialized Tools for Electrophysiology & Cell Biology Research”, 2002 Product Catalog, S. 172).
Ippei Inoue, et al., “On-Chip Culture System for Observation of Isolated Individual Cells”, Lab on a Chip, 2001, 1(1), 50-55.

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