Method for producing 3'-phosphoadenosine 5'-phosphosulfate

Details Method for producing 3'-phosphoadenosine 5'-phosphosulfate Method for producing 3'-phosphoadenosine 5'-phosphosulfate

1992-11-10

1994-12-27

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 89, C12P 1930, C12P 1932

Patent

active

053765354

ABSTRACT:
A method for producing 3'-phosphoadenosine 5'-phosphosulfate, which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of heat-stable adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, and (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of heat-stable adenosine 5'-phosphosulfate kinase; and another method for producing 3'-phosphoadenosine 5'-phosphosulfate which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of adenosine 5'-phosphosulfate kinase to produce 3'-phosphoadenosine 5'-phosphosulfate and adenosine 5'-diphosphate, and (3) converting said adenosine 5'-diphosphate into adenosine 5'-triphosphate in the presence of a phosphate donor and an enzyme capable of converting adenosine 5'-diphosphate into adenosine 5'-triphosphate are disclosed.

REFERENCES:
patent: 4164444 (1979-08-01), Whitesides et al.
patent: 4266048 (1981-05-01), Horwitz et al.
R. Cherniak et al., "Synthesis of Adenylyl Sulfate and Adenylyl Sulfate 3'-Phosphate", The Journal of Biological Chemistry, vol. 239, No. 9, Sep. 1964, pp. 2986-2990.
"Methods in Enzymology", Edited by S. P. Colowick, N. O. Kaplan, P. W. Robbins, vol. 5, Academic Press, Inc., New York and London, (1962) p. 964.
S. S. Singer, "Enzymatic Sulfation of Steroids. VI. A Simple, Rapid Method for Routine Enzymatic Preparation of 3'-Phosphoadenosine-5'-Phosphosulfate", Anal. Biochem., vol. 96, (1979) pp. 34-38.

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