Method for producing...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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Reexamination Certificate

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08034584

ABSTRACT:
A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-αβ-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-αβ-diester through a peptide bond.

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A. Schmid, et al., “Industrial Biocatalysis Today and Tomorrow,” Nature, vol. 409, Jan. 11, 2001, pp. 258-268.
Lee, et al., Effect of Methanol on the Synthesis of Aspartame Precursor Catalysed byPseudomonasAeruginosa Elastase, Biotechnology Letter, 1992, 14:779-784.

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